KARS Antibody

• Catalog Number: orb1270384
• Category: Antibodies
• Description: KARS Antibody
• Target: KARS
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
• Immunogen: This KARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the N-terminal region of human KARS.
• UniProt ID: Q15046
• MW: 68 kDa
• Tested applications: FC, WB
• Application notes: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Lysine--tRNA ligase, Lysyl-tRNA synthetase, LysRS,
• Note: For research use only
• NCBI: Q15046

Western blot analysis in MCF-7 cell line lysates (35 ug/lane).

Western blot analysis of KARS using rabbit polyclonal KARS Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KARS gene.

Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.