KANK1 Antibody

• Catalog Number: orb1925616
• Category: Antibodies
• Description: KANK1 Antibody
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1,k
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• UniProt ID: Q14678
• MW: 147289 Da
• Tested applications: IHC-P, WB
• Dilution range: IHC-P - 1:100-500, WB - 1:2000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: ANKRD15, KANK, KIAA0172
• Research Area: Cancer Biology, Signal Transduction
• Note: For research use only

All lanes: Anti-KANK1 Antibody at 1:2000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 147 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining KANK1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.