Kanamycin sulfate

SKU: orb1310286

Description

Kanamycin sulfate is an aminoglycoside antibiotic that inhibits bacterial protein synthesis by binding to the 30S ribosomal subunit. It is widely used in molecular biology for selection in cell culture and in research to study bacterial infections in both in vitro and in vivo models.

Research Area

Infectious Disease & Virology

Key Properties

• CAS Number: 25389-94-0
• MW: 582.58
• Purity: 99.63%
• Formula: C₁₈H₃₈N₄O₁₅S
• SMILES: OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• Target: Antibiotic,ribosome,Antibacterial
• Solubility: DMSO:Insoluble;H2O:100 mg/mL (171.65 mM)

Bioactivity

• Target IC50:
  Kirchbergand:1 µg/mL (MIC)|various strains of mycobacteria:1-5 μg/mL (MIC)
• In Vivo:
  METHODS: To detect ototoxicity, Kanamycin sulfate (800 mg/kg) was administered subcutaneously to C57BL/6J mice twice daily for fifteen days. RESULTS: Different ototoxic regimens were evaluated by ABR and DPOAE measurements before and after treatment. Sensory cell loss was quantified by counting hair cells in the cochlea. The study demonstrated that the Kanamycin ototoxicity regimen was safe, well tolerated, and detected early significant changes in hearing thresholds by DPOAE in a small group of mice.
• In Vitro:
  METHODS: Human cell lines HEK293, OVCAR8 and CA46 were treated with Kanamycin sulfate (0-1 mM) and cell viability was measured using the CCK-8 assay. RESULTS: Kanamycin showed no cytotoxic effects in any of the cell lines. METHODS: Mycobacteria H37Rv, H2, H37RvR-PAS, Ravenel, Kirchbergand and BCG were treated with Kanamycin sulfate (0.1-100 µg/mL) for 2 weeks and tested for antibacterial activity. RESULTS: Kanamycin showed good antimicrobial activity against various Mycobacterium species with MICs of 1 µg/mL and 5 µg/mL for Kirchbergand strains, respectively.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

16S ribosome, 30S ribosome, Antibiotic, Bacterial, bovine tubercle bacilli, Inhibitor, mycobacteria, inhibit, K. pneumonia, Kanamycin sulfate, Kanamycin Sulfate, Kanamycin Monosulfate, Kanamycin monosulfate, Kanamycin, Kanamycin A, Kanamycin A Monosulfate, Kanamycin A monosulfate, Ophtalmokalixan, pneumonia, streptomycin, tuberculosis

Compound Identifiers

PubChem CID

441374