Jun B (phospho Ser259) rabbit pAb

• Catalog Number: orb764226
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Jun B (phospho-Ser259)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human JunB around the phosphorylation site of Ser259. AA range:226-275
• MW: 33
• Tested applications: ELISA, IHC-P, IP, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-JUNB antibody, anti-Transcription factor jun-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Phospho-Jun B (S259) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using JunB (Phospho-Ser259) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells, using JunB (Phospho-Ser259) Antibody. The lane on the right is blocked with the phospho peptide.