JUN Antibody (Center T93)

SKU: orb1432907

Description

Rabbit polyclonal antibody to JUN (T93).

Images & Validation

• Tested Applications: IF, WB
• Dilution range: IF: 1:10~50, WB: 1:1000
• Reactivity: Human
• Predicted Reactivity: Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB30542
• Immunogen: Synthetic Peptide
• Target: JUN
• Molecular Weight: 35676

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, JUN

Fluorescent confocal image of U251 cell stained with JUN Antibody (Center T93). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with JUN primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). JUN immunoreactivity is localized to Nucleus significantly.

JUN Antibody (Center T93) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the JUN antibody detected the JUN protein (arrow).

Quick Database Links

Gene Symbol

JUN

UniProt

P05412

RefSeq (Protein):NP_002219.1