JSH-23

SKU: orb1224075

Description

JSH-23 is an inhibitor of NF-κB transcriptional activity with IC50 of 7.1 μM.(In Vitro):JSH-2 (1-300 μM; 24 hours) at <100 μM does not show significant cytotoxic effects on the RAW 264.7 cells.Nuclear amount of NF-κB p65 is markedly increased upon exposure to LPS for 1 h. Treatment of JSH-23 (30 μM; 1 hours) to LPS- stimulated RAW 264.7 cells decreases nuclear content of NF-κB p65 in a dose-dependent manner.(In Vivo):JSH-23 (1 mg/kg, 3 mg/kg; orally administered; daily; for 2 weeks) significantly reverses the nerve conduction and nerve blood flow deficits seen in diabetic rats.

Key Properties

• CAS Number: 749886-87-1
• MW: 240.34
• Purity: >98% (HPLC)
• Formula: C₁₆H₂₀N₂
• SMILES: NC1=CC(C)=CC=C1NCCCC2=CC=CC=C2
• Target: NF-κB
• Solubility: Ethanol: 20 mg/mL (83.21 mM); DMSO: 48 mg/mL (199.71 mM)

Bioactivity

• In Vivo:
  JSH-23 (1 mg/kg, 3 mg/kg; orally administered; daily; for 2 weeks) significantly reverses the nerve conduction and nerve blood flow deficits seen in diabetic rats. Animal model: Male Sprague Dawley diabetic rats (250-270 g). Dosage: 1 mg/kg, 3 mg/kg. Administration: Orally administered; daily; for 2 weeks. Result: Produced significant improvement in motor nerve conduction velocity (MNCV).
• In Vitro:
  JSH-2 (1-300 μM; 24 hours) at <100 μM does not show significant cytotoxic effects on the RAW 264.7 cells. Nuclear amount of NF-κB p65 is markedly increased upon exposure to LPS for 1 h. Treatment of JSH-23 (30 μM; 1 hours) to LPS- stimulated RAW 264.7 cells decreases nuclear content of NF-κB p65 in a dose-dependent manner. Cell Cytotoxicity Assay Cell line: Macrophages RAW 264.7 Concentration: 1, 3, 10, 30, 100, 300 μM. Incubation time: 24 hours. Result: Did not show significant cytotoxic effects at <100 μM. Western blot analysis. Cell line: Macrophages RAW 264.7 with LPS-stimulated Concentration: 30 μM. Incubation time: 1 hour. Result: Decreased nuclear content of NF-κB p65 in a dose-dependent manner, corresponding to 49±4% inhibition at 3 μM, 75±7% at 10 μM and 95±8% at 30 μM.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NF-κB Activation Inhibitor II

Quick Database Links

Gene Symbol

NF-κB