JSH-23

SKU: orb1307077

Description

JSH-23 is a cell-permeable inhibitor that selectively blocks NF-κB-dependent transcriptional activity (IC50=7.1 µM) without preventing IκBα degradation. This antioxidant compound is used in vitro and in vivo to study inflammatory signaling pathways and oxidative stress-related mechanisms.

Research Area

Signal Transduction

Key Properties

• CAS Number: 749886-87-1
• MW: 240.34
• Purity: 99.67% (May vary between batches)
• Formula: C₁₆H₂₀N₂
• SMILES: Cc1ccc(NCCCc2ccccc2)c(N)c1
• Target: NF-κB
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (20.8 mM);Ethanol:16 mg/mL (66.57 mM);DMSO:255 mg/mL (1061 mM)

Bioactivity

• Target IC50:
  NF-κB (RAW 264.7 cells):7.1 μM|U-87MG cells:59.39 μM|U251MG cells:56.21μM|NF-κB:7.1 μM
• In Vivo:
  METHODS: To investigate the role in diabetic neuropathy, JSH-23 (1-3 mg/kg) was administered orally to streptozotocin-induced diabetic rats once daily for two weeks. RESULTS: JSH-23 treatment significantly reversed nerve conduction and nerve blood flow deficits in diabetic animals. The treatment also partially corrected the reduced mechanical pain threshold.JSH-23 treatment inhibited nuclear translocation of the p65/p50 subunit in the sciatic nerve.
• In Vitro:
  METHODS: Bone marrow macrophage BMMs were treated with JSH-23 (0.78-200 µM) for 48-96 h. Cell viability was measured by CCK-8 assay. RESULTS: JSH-23 had no detectable toxic effects at concentrations below 50 µM. METHODS: Macrophages RAW 264.7 harboring the reporter gene pNF-κB-SEAP-NPT were treated with LPS (1 µg/mL) and JSH-23 (1-30 µM) for 16 days, and SEAP expression was assayed to reflect NF-κB transcriptional activity. RESULTS: JSH-23 inhibited LPS-induced SEAP expression in a dose-dependent manner by 23±3% at 3 µM, 68±3% at 10 µM, and 103±4% at 30 µM. JSH-23 inhibited NF-κB transcriptional activity.
• Cell Research:
  Macrophages RAW 264.7 are incubated with various concentrations of JSH-23 compound for 24 h. The cells are treated with WST-1 solution and absorbance is measured at 450 nm.(Only for Reference)

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, JSH 23, JSH23, JSH-23, inhibit, NF-κB, NFκB, NFkB, NF-kB, Nuclear factor-κB, Nuclear factor-kappaB

Compound Identifiers

PubChem CID

16760588