JNK-IN-8

SKU: orb1305191

Description

JNK-IN-8 is a potent and irreversible inhibitor of JNK isoforms 1, 2, and 4 with single-digit nM IC50 values. It demonstrates high selectivity and is widely used in research to investigate JNK signaling pathways in cellular models of stress response, inflammation, and apoptosis, both in vitro and in vivo.

Research Area

Cell Biology, Neuroscience

Key Properties

• CAS Number: 1410880-22-6
• MW: 507.6
• Purity: >98%
• Formula: C₂₉H₂₉N₇O₂
• SMILES: CN(C)CC=CC(=O)Nc1cccc(c1)C(=O)Nc1ccc(Nc2nccc(n2)-c2cccnc2)c(C)c1
• Target: Kinase
• Solubility: Soluble in DMSO (45 mg/ml)

Bioactivity

• Target IC50:
  JNK2:18.7 nM|JNK:1 nM|JNK1:4.7 nM
• In Vivo:
  JNK-IN-8 (10 mM) effectively inhibits the phosphorylation of c-Jun stimulated by IL-1β in IL-1R cells. It displays significant selectivity for the 1,4-bis(phenylamino) and 1,3-aminobenzoic acid structural regions compared to imatinib, covalently binding to the Cys154 target via an N,N-dimethylbutenamide linkage. JNK-IN-8 inhibits the phosphorylation of c-Jun in HeLa (EC50: 486 nM) and A375 (EC50: 338 nM) cells. Its binding with PIK3C3, IRAK1, PIP5K3, and PIP4K2C significantly enhances selectivity and elimination rates. JNK-IN-8 inhibits JNK2 through Cys116.
  Read more...
• Cell Research:
  JNK-IN-8 is dissolved in DMSO and stored, and then diluted with appropriate media before use. HEK-293 cells stably expressing Interleukin Receptor 1 (HEK293-IL1R) are cultured in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% FBS, 2 mM glutamine and 1×antimycotic/antibiotic solution. Cells are serum starved for 18 h before incubation with DMSO or JNK-IN-8, stimulated with 2 μM Anisomycin for 1h and lysates are clarified by centrifugation for 10 min at 16000 g and 4°C.
  Read more...

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

JNK Inhibitor XVI

Compound Identifiers

PubChem CID

76516568