JNK-IN-8

SKU: orb1305191

Description

JNK-IN-8

Research Area

Signal Transduction

Key Properties

• CAS Number: 1410880-22-6
• MW: 507.59
• Purity: 99.59%
• Formula: C₂₉H₂₉N₇O₂
• SMILES: CN(C)CC=CC(=O)Nc1cccc(c1)C(=O)Nc1ccc(Nc2nccc(n2)-c2cccnc2)c(C)c1
• Target: c-Kit,JNK
• Solubility: Ethanol:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.94 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:56 mg/mL (110.33 mM)

Bioactivity

• Target IC50:
  JNK2:18.7 nM|JNK:1 nM|JNK1:4.7 nM
• In Vivo:
  JNK-IN-8 (10 mM) effectively inhibits the phosphorylation of c-Jun stimulated by IL-1β in IL-1R cells. It displays significant selectivity for the 1,4-bis(phenylamino) and 1,3-aminobenzoic acid structural regions compared to imatinib, covalently binding to the Cys154 target via an N,N-dimethylbutenamide linkage. JNK-IN-8 inhibits the phosphorylation of c-Jun in HeLa (EC50: 486 nM) and A375 (EC50: 338 nM) cells. Its binding with PIK3C3, IRAK1, PIP5K3, and PIP4K2C significantly enhances selectivity and elimination rates. JNK-IN-8 inhibits JNK2 through Cys116.
• Cell Research:
  JNK-IN-8 is dissolved in DMSO and stored, and then diluted with appropriate media before use. HEK-293 cells stably expressing Interleukin Receptor 1 (HEK293-IL1R) are cultured in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% FBS, 2 mM glutamine and 1×antimycotic/antibiotic solution. Cells are serum starved for 18 h before incubation with DMSO or JNK-IN-8, stimulated with 2 μM Anisomycin for 1h and lysates are clarified by centrifugation for 10 min at 16000 g and 4°C.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cKit, Kit (V559D), Kit (V559D,T670I), inhibit, JNK, JNK IN 8, JNK Inhibitor XVI, JNK1, JNK2, JNK3, JNKIN8, JNK-IN-8, Inhibitor

Compound Identifiers

PubChem CID

76516568