JNK-IN-7

SKU: orb1304014

Description

JNK-IN-7

Research Area

Signal Transduction

Key Properties

• CAS Number: 1408064-71-0
• MW: 493.56
• Purity: 99.58% (May vary between batches)
• Formula: C₂₈H₂₇N₇O₂
• SMILES: CN(C)CC=CC(=O)Nc1cccc(c1)C(=O)Nc1ccc(Nc2nccc(n2)-c2cccnc2)cc1
• Target: JNK
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.05 mM);DMSO:28.88 mg/mL (58.51 mM)

Bioactivity

• Target IC50:
  JNK3:0.75 nM|JNK2:1.99nM|JNK1:1.54 nM
• In Vitro:
  JNK-IN-7 is a relatively selective JNK inhibitor in cells, binding to JNK1, 2, 3, IRAK1 (IC50=14.1 nM), YSK4 (IC50=4.8 nM), ERK3 (IC50=22 nM), PIK3C3, PIP5K3, and PIP4K2C. In HCT116 cells, TNF stimulation for 24 and 48 hours significantly decreases divalent metal-ion transporter 1 (DMT1) expression, a process that JNK-IN-7 can notably counteract.
• Cell Research:
  JNK-IN-7 is prepared in DMSO and stored, and then diluted with appropriate medium before use. Intestinal epithelial cell line (HCT116) is cultured in DMEM medium, supplemented with 10% heat-inactivated fetal bovine serum (FBS), penicillin (100 U/mL) and streptomycin (100 g/mL), 2 mM L-gentamycin, and 50 μM 2-ME. These cells are stimulated with TNF (20 ng/mL), LPS (100 ng/mL), and IFN-γ (20 ng/mL), respectively. After 24 or 48 h of culture, cells are harvested followed by extraction of total RNA, and the levels of DMT1 mRNA are analyzed by qRT-PCR. To determine the mechanisms of TNF involved in regulating DMT1 expression, JNK-IN-7 (1 μM), NF-κB inhibitor (BAY 11-7082, 1 μM), and caspase-3/8 inhibitor (Z-DEVD-FMK, 50 μM) are also added into the culture medium. After 48 h of culture, cells are then collected to detect the expression of DMT1 by qRT-PCR.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, JNKIN7, JNK-IN-7, JNK3, JNK2, JNK1, JNK, JNK IN 7, JNK inhibitor, Inhibitor

Compound Identifiers

PubChem CID

76516567