JLP (SPAG9) Antibody (N-term)

SKU: orb1929391

Description

JLP (SPAG9) Antibody (N-term)

Research Area

Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: IHC-P-Leica - 1:250, WB - 1:2000
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 146205 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Immunohistochemical analysis on paraffin-embedded human testis tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:250) for 15 min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.

Anti-JLP (SPAG9) Antibody (N-term) at 1:2000 dilution + PC-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 146 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

O60271