JIP-1 (phospho Thr103) rabbit pAb

• Catalog Number: orb770923
• Category: Antibodies
• Description: Rabbit polyclonal antibody to JIP-1 (phospho-Thr103)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human JIP1 around the phosphorylation site of Thr103. AA range:69-118
• MW: 113
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-MAPK8IP1 antibody, anti-IB1 antibody, anti-JI
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of COLO cells using Phospho-JIP-1 (T103) Polyclonal Antibody.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of NIH/3T3 cells, using JIP1 (Phospho-Thr103) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from COLO205 cells treated with Serum 20% 15', using JIP1 (Phospho-Thr103) Antibody. The lane on the right is blocked with the phospho peptide.