Jervine

SKU: orb1304279

Description

Jervine is a steroidal alkaloid that acts as a potent inhibitor of the Smoothened (SMO) receptor, a key component of the Hedgehog signaling pathway. By binding to and inhibiting SMO, it prevents the activation of GLI transcription factors and downstream target genes. This mechanism makes it a valuable research tool for studying Hedgehog signaling in cancer, developmental biology, and in both cellular and animal models.

Research Area

Pharmacology & Drug Discovery, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 469-59-0
• MW: 425.6
• Purity: >98%
• Formula: C₂₇H₃₉NO₃
• SMILES: [H][C@@]12NC[C@@H](C)C[C@H]1O[C@]1(CC[C@]3([H])C(C(=O)[C@@]4([H])[C@@]3([H])CC=C3C[C@@H](O)CC[C@]43C)=C1C)[C@@H]2C
• Target: GPCR
• Solubility: Soluble in DMSO (up to 5 mg/ml), in DMF (up to 6 mg/ml), or in Ethanol (up to 20 mg/ml).

Bioactivity

• Target IC50:
  Hedgehog:500-700 nM (IC50)
• In Vivo:
  Embryos exposed to jervine develop craniofacial abnormalities related to those found in Shh mutant embryos. The lower jaw of these embryos is severely reduced in size, often leading to loss of the incisors. Treatment of pregnant mice with the hedgehog inhibitor jervine induces jaw defects in the embryos similar to those found in Prx1 / Prx2 / embryos. The distal part of the jaw is reduced in size, and both incisors are absent.
• In Vitro:
  Compared to controls, chondrocyte cultures exposed to 25 μg/ml jervine shows an apparent reduction in cell number but no differences in cellular morphology or relative intensity of alcian blue staining.
• Cell Research:
  Cells are grown in Eagle's basal medium supplemented with 10% fetal bovine serum and 0.25 mg/ml gentamicin sulfate. Cells are seeded onto 35-mm tissue culture plates at a density of 1×104 celldplate. The plated cells are incubated for 24 hours prior to treatment in a humidified incubator at 37 "C in an atmosphere of 5% CO2 in air. Drugs are then added: jervine to a final concentration of 5, 10, or 25 μg/ml; retinoic acid at 0.03, 0.30, or 3.0 pg/ml. After a final 48 hours of incubation, media are removed, the cultures are rinsed three times with phosphate-buffered saline (PBS) pH 7.4, and the cells are harvested into 0.5 ml of 0.1% (w/v) trypsin which contained 0.1% EDTA (w/v) in PBS. Each sample is mixed with 9.5 ml Isoton Ⅱ, and cell numbers are determined by using a Coulter Counter. (Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

11-Ketocyclopamine

Compound Identifiers

PubChem CID

10098