Jervine

SKU: orb1223122

Description

The biological activity of Jervine is mediated via its interaction with the 7 passes transmembrane protein Smoothened. Jervine binds with and inhibits smoothened, which is an integral part of the Hedgehog signaling pathways. With smoothened inhibited, the GLI1 transcription cannot be activated and Hedgehog target genes cannot be transcribed.

Key Properties

• CAS Number: 469-59-0
• MW: 425.61
• Purity: >98% (HPLC)
• Formula: C₂₇H₃₉NO₃
• SMILES: O=C1C2=C(C)[C@]3(CC[C@H]2[C@@H]2CC=C4C[C@H](CC[C@]4(C)[C@@H]12)O)O[C@@H]1C[C@@H](CN[C@H]1[C@H]3C)C
• Target: Hedgehog| Smoothened
• Solubility: DMSO : 1 mg/mL2.35 mM

Bioactivity

• In Vivo:
  Jervine (orally; 50-400 mg/kg) exerts 50.4-73.5% anti-inflammatory effects in carrageenan induced paw edema. Animal model: Male Sprague Dawley rats (180-200 g). Dosage: 50, 100, 200 and 400 mg/kg. Administration: Orally. Result: Exerted 50.4-73.5% anti-inflammatory effects in carrageenan induced paw edema.
• In Vitro:
  Jervine (40 μM; 6, 12 and 24 hours) inhibits Akt phosphorylation. Jervine (40 μM; 2 hours) inhibits NF-jB activation decreased COX-2 overexpression and induced apoptosis. Western blot analysis. Cell line: HEL and TF1a cells. Concentration: 40 μM. Incubation time: 6, 12 and 24 hours. Result: Inhibited Akt phosphorylation.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

11-Ketocyclopamine

Quick Database Links

Gene Symbol

Hedgehog| Smoothened