JAM-A, Human, mAb M.Ab.F11 Antibody

SKU: orb1108962

Description

Mouse monoclonal antibody to JAM-A

Images & Validation

• Tested Applications: FC, Functional Studies, IA, IF, IP, PS, WB
• Dilution Range: For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, dilutions have to be optimized in user's experimental setting.
• Reactivity: Human
• Application Notes:
  FC: Antibody M.Ab. F11 stains the extracellular domain of JAM-A protein name. Cells were incubated with 5 µg/ml of mAb in 0.1%BSA/PBS. As negative control an isotype-matched antibody was used (Ref.4) W: A non-reduced and reduced sample treatment and SDS-Page was used. The band sizes- are 32 and 35kDa (Ref. 1). FS: Antibody M.Ab. F11 functions as anagonist resulting in platelet aggregation at concentrations of approximately 5 µg/ml (Ref.1).

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Immunogen: Human platelet membranes
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.
• Buffer/Preservatives: Quantities >500 µg will be sent without preservatives and are carrier-free.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

JAM-1, platelet F11 receptor, F11R, platelet adhesion molecule, CD321

Quick Database Links

UniProt

Q9Y624