IWP 4

SKU: orb1223077

Description

IWP-4 is a novel potent inhibitor of Wnt/β-catenin signaling.

Key Properties

• CAS Number: 686772-17-8
• MW: 496.6
• Purity: >98% (HPLC)
• Formula: C₂₃H₂₀N₄O₃S₃
• SMILES: COc1ccccc1-n1c(SCC(=O)Nc2nc3ccc(C)cc3s2)nc2CCSc2c1=O
• Target: GluR
• Solubility: DMSO : 5 mg/mL. 10.07 mM

Bioactivity

• In Vitro:
  IWP-4 is a small molecule Wnt inhibitor with an IC50 of 25 nM. IWP-4 induces the expression of cardiac markers, including cardiac troponin I (CTNI) and cardiac myosin heavy chain bright cells (MYHhi +). IWP-4 also results in the appearance of beating foci (0.44±0.10 SEM beats per second), which is absent in all cultures not receiving IWP-4. Further, flow cytometric analysis shows that there are significantly more MYHlo + cells in IWP-4 treated cultures (P<0.0002) compare with untreated cultures at day 16, being 17.0±1.3 SD% and 5.4±1.4 SD%, respectively. Quantification of NKX2-5 protein expression shows that 63% (481/817) of IWP-4 treated cells display nuclear NKX2-5 expression. Mesenchymal precursor cells (MPCs) treated with IWP-4 show no significant changes in the expression of AXIN2, CTNNB1 and GSK3B as compare to osteogenic medium alone on day 7, but MPCs treated with IWP-4 express elevates levels of DKK1 and GSK3β on day 21.IWP-4 also causes a significant down regulation of SPARC and COL1A1.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IWP4 | IWP 4 | IWP-4

Quick Database Links

Gene Symbol

GluR