ITGAX Antibody (C-term)

• Catalog Number: orb1929673
• Category: Antibodies
• Description: ITGAX Antibody (C-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P20702
• MW: 127829 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50, IF - 1:10-50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CD11C
• Research Area: Immunology & Inflammation, Signal Transduction
• Note: For research use only

Flow cytometric analysis of SK-Br-3 cells using ITGAX Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence analysis of ITGAX Antibody (C-term) with paraffin-embedded human hepatocarcinoma.0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with ITGAX Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of ITGAX Antibody (C-term) in MDA-MB231 cell line lysates (35 ug/lane). ITGAX (arrow) was detected using the purified Pab.