ITGA6 Antibody (isoform 2 S1064)

SKU: orb1931487

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF - 1:200, WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB15309
• Immunogen: This ITGA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1043-1072 amino acids from isoform 2 of human ITGA6. Antigen Region: 1043-1072 aa.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Integrin alpha-6, CD49 antigen-like family member F, VLA-6, CD49f, Integrin alpha-6 heavy chain, Integrin alpha-6 light chain, Processed integrin alpha-6, Alpha6p, ITGA6

Fluorescent confocal image of HeLa cells stained with ITGA6 (isoform 2 S1064) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ITGA6 (isoform 2 S1064) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with ITGA6 Antibody (isoform 2 S1064), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-ITGA6 Antibody (isoform 2 S1064) in 293 cell line lysates (35 ug/lane). ITGA6 (arrow) was detected using the purified Pab.

Anti-ITGA6 Antibody (isoform 2 S1064) at 1:1000 dilution + SW480 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 119 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P23229

RefSeq (Protein):NP_001073286.1, NP_000201.2