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Catalog Number | orb1257160 |
---|---|
Category | Antibodies |
Description | ITGA5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 750-1000 of human ITGA5 (NP_002196.4). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 135kDa |
Target | ITGA5 |
UniProt ID | P08648 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | ITGA5, FNRA, Integrin alpha-5, Integrin Alpha 5, I Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using ITGA5 antibody (orb1257160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using ITGA5 antibody (orb1257160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
FITC |
ELISA, IF, IHC-Fr, IHC-P, WB | |
Bovine, Equine, Porcine, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, WB | |
Bovine, Canine, Equine, Mouse, Porcine, Sheep | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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