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ITGA5 Antibody
SKU: orb1257160
Featured
Description
Research Area
Cell Biology, Immunology & Inflammation, Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 750-1000 of human ITGA5 (NP_002196.4). |
| Target | ITGA5 |
| Molecular Weight | Observed: 135kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ITGA5, FNRA, Integrin alpha-5, Integrin Alpha 5, Integrin alpha-F, VLA-5, CD49e, CD49e antigen, VLA5A
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Western blot analysis of extracts of various cell lines, using ITGA5 antibody (orb1257160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using ITGA5 antibody (orb1257160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Quick Database Links
Gene Symbol
ITGA5
UniProt
UniProt Details
− No UniProt data available
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ITGA5 Antibody (orb1257160)
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