ITGA2 Antibody, KO Validated

• Catalog Number: orb1274426
• Category: Antibodies
• Description: ITGA2 Antibody, KO Validated
• Target: ITGA2
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant protein of human ITGA2
• UniProt ID: P17301
• MW: Observed: 150kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ITGA2, Alpha2 integrin, BR, CD49b antigen, CD49B,
• Note: For research use only

Western blot analysis of extracts of various cell lines, using ITGA2 antibody (orb1274426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Western blot analysis of extracts from normal (control) and ITGA2 knockout (KO) HeLa cells, using ITGA2 antibody (orb1274426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat brain using ITGA2 Antibody (orb1274426) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using ITGA2 Antibody (orb1274426) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using ITGA2 antibody (orb1274426) at dilution of 1:100. Blue: DAPI for nuclear staining.