ITA6 Antibody

SKU: orb1939421

Description

ITA6 Antibody

Research Area

Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: WB - 1:100-16000, IHC-P - 1:100-500, IF - 1:10-50, FC - 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,κ
• Molecular Weight: 126606 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-ITA6 at 1:1000 dilution + HepG2 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 127 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of ITA6 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

ITA6 Monoclonal Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

ITA6 Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ITA6 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

UniProt

P23229