Ispinesib

SKU: orb1306472

Description

Ispinesib (SB-715992) is a quinazolinone-derived, potent, and reversible inhibitor of kinesin spindle protein (KSP) with antineoplastic activity. It is widely used in cancer research to study mitotic arrest and has demonstrated efficacy in both in vitro cell-based assays and in vivo xenograft tumor models.

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 336113-53-2
• MW: 517.06
• Purity: 99.57% (May vary between batches)
• Formula: C₃₀H₃₃ClN₄O₂
• SMILES: CC(C)[C@@H](N(CCCN)C(=O)c1ccc(C)cc1)c1nc2cc(Cl)ccc2c(=O)n1Cc1ccccc1
• Target: Apoptosis,Kinesin,KSP
• Solubility: DMSO:95 mg/mL (183.73 mM);Ethanol:95 mg/mL (183.73 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (6.38 mM)

Bioactivity

• Target IC50:
  KSP (HsEg5):1.7 nM(Ki app)
• In Vivo:
  In PC-3 prostate cancer cells, Ispinesib (5 nM and 30 nM) inhibited cell proliferation and induced apoptosis by modulating the level of gene expression of signals. In breast cancer cell lines, Ispinesib (7.4 nM-600 nM) exhibited broad-spectrum inhibitory activity. In tumor cell lines (Colo205, Colo201, HT-29, M5076, Madison-109, and MX-1), Ispinesib (IC50=1.2-9.5 nM) is highly cytotoxic.
• In Vitro:
  In PC-3 prostate cancer cells, Ispinesib (5 nM and 30 nM) inhibited cell proliferation and induced apoptosis by modulating the level of gene expression of signals. In breast cancer cell lines, Ispinesib (7.4 nM-600 nM) exhibited broad-spectrum inhibitory activity. In tumor cell lines (Colo205, Colo201, HT-29, M5076, Madison-109, and MX-1), Ispinesib (IC50=1.2-9.5 nM) is highly cytotoxic.
• Cell Research:
  Cells are plated in log phase of growth in 96-well plates and treated with Ispinesib for 72 hours. Then, cell growth is measured using CellTiter-Glo, and luminescence is detected using BioTek FLx800. Data are analyzed and the IC50 value, defined as the drug concentration that results in 50% growth inhibition relative to control, is calculated.(Only for Reference)

Storage & Handling

• Storage: store at low temperature,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CK0238273, CK-0238273, CK 0238273, Apoptosis, Inhibitor, KSP (HsEg5), Kinesin, Ispinesib, inhibit, SB 715992, SB715992, SB-715992

Compound Identifiers

PubChem CID

6851740