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ISLR Antibody (C-term)
Catalog Number: orb1930782
| Catalog Number | orb1930782 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB22277 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 45997 Da |
| Target | This ISLR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 333-362 amino acids from the C-terminal region of human ISLR. |
| UniProt ID | O14498 |
| NCBI | NP_958934.1, NP_005536.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Immunoglobulin superfamily containing leucine-rich Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
ISLR Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ISLR Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ISLR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
All lanes: Anti-ISLR Antibody (C-term) at 1:1000 dilution. Lane 1: human heart lysate. Lane 2: human skeletal muscle lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
