IRS2 Antibody (C-term)

SKU: orb1928892

Description

IRS2 Antibody (C-term)

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: WB - 1:1000, IF - 1:10-50, IHC-P - 1:100-500
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 137334 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Western blot analysis of anti-IRS2 Antibody (C-term) in 293 cell line lysates (35 ug/lane). IRS2 (arrow) was detected using the purified Pab.

Anti-IRS2 Antibody (C-term) at 1:1000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 137 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IRS2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Fluorescent image of Hela cell stained with IRS2 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IRS2 primary antibody (1: 25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).IRS2 immunoreactivity is localized to Cytoplasm significantly.

Quick Database Links

UniProt

Q9Y4H2