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IRS2 Antibody (C-term)

Catalog Number: orb1928892

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1928892
CategoryAntibodies
DescriptionPurified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB15641
Tested applicationsIF, IHC-P, WB
ReactivityHuman
IsotypeRabbit IgG
Dilution rangeIF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
ConjugationUnconjugated
MW137334 Da
TargetThis IRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1309-1338 amino acids from the C-terminal region of human IRS2.
UniProt IDQ9Y4H2
NCBINP_003740.2
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesInsulin receptor substrate 2, IRS-2, IRS2
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NoteFor research use only
Expiration Date12 months from date of receipt.
IRS2 Antibody (C-term)

Western blot analysis of anti-IRS2 Antibody (C-term) in 293 cell line lysates (35 ug/lane). IRS2 (arrow) was detected using the purified Pab.

IRS2 Antibody (C-term)

Anti-IRS2 Antibody (C-term) at 1:1000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 137 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

IRS2 Antibody (C-term)

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IRS2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

IRS2 Antibody (C-term)

Fluorescent image of Hela cell stained with IRS2 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IRS2 primary antibody (1: 25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).IRS2 immunoreactivity is localized to Cytoplasm significantly.