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| Catalog Number | orb768801 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to IRS-1 (phospho-Tyr896) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Tyr896. AA range:862-911 |
| MW | 170 |
| Tested applications | ELISA, WB |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-IRS1 antibody, anti-Insulin receptor substrat Read more... |
| Note | For research use only |
Western blot analysis of KB MCF-7 using p-IRS-1 (Y896) antibody. Antibody was diluted at 1:500.
Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40', using IRS-1 (Phospho-Tyr896) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
