IRS-1 (phospho Ser307) rabbit pAb

• Catalog Number: orb764216
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IRS-1 (phospho-Ser307)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser307. AA range:274-323
• MW: 170
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-IRS1 antibody, anti-Insulin receptor substrat
• Note: For research use only

Western Blot analysis of various cells using Phospho-IRS-1 (S307) Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of VEC cells using Phospho-IRS-1 (S307) Polyclonal Antibody diluted at 1:1000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRS-1 (Phospho-Ser307) Antibody.

Immunofluorescence analysis of COS7 cells, using IRS-1 (Phospho-Ser307) Antibody. The picture on the right is blocked with the phospho peptide.