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Catalog Number | orb1537871 |
---|---|
Category | Antibodies |
Description | IRGM/LRG-47 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A 14 AA synthetic peptide near the center of the human IRGM. The immunogen is located within the last 50 AA of IRGM. |
Concentration | 1 mg/ml |
Dilution range | ELISA, IF, IHC, IHC-P (5 µg/ml), WB (1 - 2 µg/ml) |
Conjugation | Unconjugated |
Target | IRGM / LRG-47 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide |
Alternative names | IRGM, LRG-47-like protein, IFI1, IRGM1, LRG47, LRG Read more... |
Note | For research use only |
Application notes | Further information: IRGM antibody can be used for detection of IRGM by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 20 kDa Observed: 24 kDa |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence of IRGM in Human Brain cells with IRGM antibody at 20 ug/ml.
Western blot of IRGM in human brain lysate with IRGM antibody at (A) 1 and (B) 2 ug/ml.