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Catalog Number | orb1937893 |
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Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB19229 |
Tested applications | IF, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:2000, IHC-P: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 43696 Da |
Target | This IRF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-104 amino acids of human IRF9. |
UniProt ID | Q00978 |
NCBI | NP_006075.3 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Interferon regulatory factor 9, IRF-9, IFN-alpha-r Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-IRF9 Antibody (N-term) at 1:2000 dilution + NIH/3T3 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of IRF9 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
IRF9 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of IRF9 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
IRF9 Antibody (N-term) western blot analysis in mouse spleen tissue lysates (35 ug/lane). This demonstrates the IRF9 antibody detected the IRF9 protein (arrow).