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Catalog Number | orb1931422 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Target | This IRF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the N-terminal region of human IRF5. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
UniProt ID | Q13568 |
MW | 56044 Da |
Tested applications | FC, IF, WB |
Dilution range | IF: 1:10~50, WB: 1:1000, FC: 1:10~50 |
Antibody Type | Primary Antibody |
Clone Number | RB17419 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Interferon regulatory factor 5, IRF-5, IRF5 |
Note | For research use only |
NCBI | NP_001092097.2, NP_001092100.1, NP_001092099.1, NP_001229381.1, NP_116032.1 |
IRF5 Antibody (N-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of IRF5 Antibody (N-term) with A549 cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of anti-IRF5 Antibody (N-term) in Ramos cell line lysates (35 ug/lane). IRF5 (arrow) was detected using the purified Pab.