IRF3 Antibody / Interferon regulatory factor 3

SKU: orb1151863

Description

Mouse monoclonal antibody to IRF3

Research Area

Epigenetics, Immunology, Infectious Diseases

Images & Validation

• Tested Applications: FACS, IF, WB
• Dilution Range: Western blot: 2-4ug/ml,Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells
• Reactivity: Human
• Application Notes:
  Optimal dilution of the IRF3 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: PCRP-IRF3-3B2
• Immunogen: Recombinant full-length human IRF3 protein was used as the immunogen for the IRF3 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human HeLa cell lysate using IRF3 antibody (clone PCRP-IRF3-3B2). Predicted molecular weight ~47 kDa.

SDS-PAGE analysis of purified, BSA-free IRF3 antibody (PCRP-IRF3-3B2) as confirmation of integrity and purity.

FACS staining of PFA-fixed human HeLa cells with IRF3 antibody (blue, clone PCRP-IRF3-3B2) and isotype control (red).

Immunofluorescent staining of PFA-fixed human HeLa cells using IRF3 antibody (green, clone PCRP-IRF3-3B2) and phalloidin (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using IRF3 antibody (clone PCRP-IRF3-3B2). These results demonstrate the foremost specificity of the PCRP-IRF3-3B2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Quick Database Links

UniProt

Q14653