IRF-4 rabbit pAb Antibody

SKU: orb765519

Description

Rabbit polyclonal antibody to IRF-4

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human IRF4. AA range:281-330
• Molecular Weight: 45
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-IRF4 antibody, anti-MUM1 antibody, anti-Interferon regulatory factor 4 antibody, anti-IRF-4 antibody, anti-Lymphocyte-specific interferon regulatory factor antibody, anti-LSIRF antibody, anti-Multiple myeloma oncogene 1 antibody, anti-NF-EM5 antibody

Western Blot analysis of various cells using IRF-4 Polyclonal Antibody.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western blot analysis of lysates from HeLa and Jurkat cells, using IRF4 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HT-29 cells using IRF4 antibody.