IRAK4 Antibody (N-term)

• Catalog Number: orb1928873
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This IRAK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-36 amino acids from the N-terminal region of human IRAK4.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9NWZ3
• MW: 51530 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Antibody Type: Primary Antibody
• Clone Number: RB2347
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Interleukin-1 receptor-associated kinase 4, IRAK-4
• Note: For research use only
• NCBI: NP_057207.2, NP_001138730.1, NP_001138728.1, NP_001107654.1, NP_001138729.1

Western blot analysis of IRAK4 (arrow) using rabbit polyclonal IRAK4 Antibody (R20). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IRAK4 gene.

All lanes: Anti-IRAK4 Antibody (N-term) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 52 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.