IRAK3 Antibody

SKU: orb1247357

Description

IRAK3 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 65kDa band observed in Rat Liver lysates (calculated MW of 67.8kDa according to NP_082955.2). An approx 70kDa is observed in Mouse Bone Marrow lysates but this band disappears in lysates from the knock-out mouse. Recommended concentration: 0.05-0.2µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-GHSYGSKPMEKR
• Target: IRAK3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IRAK3, interleukin-1 receptor-associated kinase 3, ASRT5, IRAK-M, IRAKM, interleukin-1 receptor-associated kinase M

orb1247357 (0.05 μg/ml) staining of Rat Liver lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected with chemiluminescence.

orb1247357 (0.5 μg/ml) staining of Mouse Bone Marrow (wildtype first lane, KO second lane) lysates (35 μg protein in RIPA buffer). Primary incubation was overnight at 4C Detected with chemiluminescence.

Quick Database Links

Gene Symbol

IRAK3

UniProt

Q8K4B2

RefSeq (Protein):NP_082955.2