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IRAK3 Antibody (N-term)
Catalog Number: orb1928874
| Catalog Number | orb1928874 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This IRAK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-77 amino acids from the N-terminal region of human IRAK3. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | Q9Y616 |
| MW | 67767 Da |
| Tested applications | IHC-P, WB |
| Dilution range | WB: 1:500, IHC-P: 1:100 |
| Antibody Type | Primary Antibody |
| Clone Number | RB2344 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Interleukin-1 receptor-associated kinase 3, IRAK-3 Read more... |
| Note | For research use only |
| NCBI | NP_009130.2, NP_001135995.1 |
All lanes: Anti-IRAK3 Antibody (N-term) at 1:500 dilution. Lane 1: Raji whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: Mouse spleen tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis on paraffin-embedded Human tonsil tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
