Ipafricept Antibody

SKU: orb1980408

Description

Ipafricept (OMP-54F28) is a Wnt pathway inhibitor composed of a human frizzled-8 receptor decoy fused to an IgG1 Fc fragment. It acts by sequestering Wnt ligands, demonstrating antitumor activity in preclinical studies of solid tumors, including in vivo models of ovarian and pancreatic cancers.

Research Area

Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 1391727-24-4
• MW: 41.12 kDa
• Purity: 99.2% (SDS-PAGE); 97.4% (SEC-HPLC)
• Target: Wnt/beta-catenin

Bioactivity

• In Vivo:
  Ipafricept administered intraperitoneally at a dose of 10 mg/kg per week or 25 mg/kg every 2 weeks for 42 days in combination with Gemcitabine (50 mg/kg per week or 5 mg/kg per week) and Nab-paclitaxel (10 mg/kg per week) significantly enhanced tumor growth inhibition. In a mouse model of ovarian cancer xenografts, Ipafricept administered intraperitoneally at a dose of 45 mg/kg every 2 weeks for 42 days, in combination with Nab-paclitaxel (7.5 mg/kg/week), demonstrated enhanced anti-tumor activity over Carboplatin (30 mg/kg/week). In a mouse model of total skin injury, Ipafricept administered subcutaneously at a dose of 10 mg/kg on days 0 and 3 was able to compete with RL-QN15 for binding and reduce the interaction of RL-QN15 with FZD8, thereby counteracting its activation of the Wnt/β-catenin signaling pathway.
• In Vitro:
  Ipafricept inhibited the proliferative and migratory effects of the peptide RL-QN15 on human embryonic stem cells (hESCs) after treatment with 10 μg/mL for 4 hours. When Ipafricept was treated with 10 μg/mL for 0-48 hours, it reversed the RL-QN15-induced activation of the Wnt/β-catenin signaling pathway, which in turn reversed the effects of RL-QN15 on the proliferation, migration and stemness of hESCs.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

OMP54F28, OMP-54F28, FZD8Fc, FZD8-Fc, bcatenin, betacatenin, beta-catenin, Wnt, Wnt/b-catenin, Wnt/betacatenin, Wnt/β-catenin, βcatenin

Quick Database Links

Gene Symbol

Wnt/beta-catenin