IPAF/NLRC4 Antibody

• Catalog Number: orb1538540
• Category: Antibodies
• Description: IPAF/NLRC4 Antibody
• Target: IPAF / NLRC4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Purification: Peptide Column Affinity Chromatography
• Immunogen: A synthetic peptide corresponding to amino acids near the C-Terminus of human Ipaf. The immunogen is located within the last 50 amino acids of Ipaf.
• Tested applications: ELISA, ICC, IF, IHC, IHC-P, WB
• Dilution range: ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB (0.5 - 2 µg/ml)
• Application notes: Further information: Ipaf polyclonal antibody can be used for the detection of Ipaf by Western blot at 1 and 2 ug/mL. A 110 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 10 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: 110 kDa
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NLRC4, CARD12, CLANA, CLANB, CLANC, CLAND, CLAN, C
• Note: For research use only

Immunofluorescence of Ipaf in THP1 cells with Ipaf antibody at 10 ug/ml.

Western blot analysis of Anti-IPAF antibody (orb1538540, 2 µg/ml; 15 µg of lysate per lane). Lane 1: THP-1 Cell lysate. Antibody produced band at ~116 kDa.