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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 3.3-1G1-2G7-2F6 |
| Immunogen | Produced in BALB/c mice using highly pure recombinant Human IP-10 as the immunizing antigen. This antibody was purified from cell culture by Protein A affinity chromatography. |
| Target | CXCL10 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CXCL10, INP10, SCYB10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Gamma-IP10, C7, IFI10, IP-10, crg-2, mob-1, gIP-10
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To a sandwich ELISA (assuming 100 ul/well), a concentration of 1.0-2.0 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant Human IP-10 when used with Biotinylated Antigen Affinity Purified anti-Human IP-10 (orb1272429) as the detection antibody at a concentration of approximately 0.25 ug/ml.
To detect Human IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml (non-reducing) or at a concentration of 1.0-2.0 ug/ml (reducing). When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IP-10 is 0.20-0.40 ng/lane under non-reducing conditions and 2.00-4.00 ng/lane under reducing conditions.
To detect Human IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml (non-reducing) or at a concentration of 1.0-2.0 ug/ml (reducing). When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IP-10 is 0.20-0.40 ng/lane under non-reducing conditions and 2.00-4.00 ng/lane under reducing conditions.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CXCL10 Antibody (orb1272640)
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