Ionomycin

SKU: orb2279136

Description

Ionomycin is a calcium ionophore antibiotic used in research to elevate intracellular Ca²⁺ levels, thereby activating calcium-dependent processes like apoptosis and enzyme activity. It is widely applied in both in vitro and in vivo studies to investigate T-cell activation, signal transduction, and programmed cell death.

Research Area

Cell Biology

Key Properties

• CAS Number: 56092-81-0
• MW: 709.0
• Purity: >99%
• Formula: C₄₁H₇₂O₉
• SMILES: [H][C@@]1(C[C@H](O)[C@H](C)[C@H](O)[C@H](C)\C=C\C[C@@H](C)C[C@@H](C)C(\O)=C\C(=O)[C@@H](C)C[C@@H](C)C[C@H](C)CCC(O)=O)CC[C@](C)(O1)[C@@]1([H])CC[C@](C)(O1)[C@@H](C)O
• Target: Ionophore
• Solubility: Soluble in DMSO (up to 10 mg/ml) or in Ethanol (30 mg/ml).

Bioactivity

• In Vivo:
  METHODS: To investigate the activation of immune cells by Ionomycin, PMA and Ionomycin were used to activate CD4+CD25- T cells in mice to generate inductive regulatory T cells (iTreg), and their functions were tested in a mouse model of inflammatory bowel disease (IBD). RESULTS: iTreg cells activated by Ionomycin could significantly alleviate the symptoms of IBD mouse models, including weight loss and reduced disease activity scores. METHODS: To study the role of calcium signaling in embryonic development, Ionomycin was added to the mouse embryo culture medium. RESULTS: Calcium signaling plays a key role in embryonic cell migration and morphogenesis.
• In Vitro:
  METHODS: LCLC 103H cells were treated with Ionomycin and analyzed using DNA and protein. RESULTS: Ionomycin induces DNA fragmentation and PARP lysis into an 85-kDa fragment of typical caspase-mediated apoptosis.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CalciumChannel, Calcium Channel, Calcium ionophore, Ionomycin

Compound Identifiers

PubChem CID

6912226