CCL2/MCP-1 Human Monoclonal Antibody

SKU: orb2960360

Description

CCL2/MCP-1 Human Monoclonal Antibody is an unconjugated monoclonal antibody. It shows reactivity with Human samples. It is suitable for use in ELISA, FC, NeA applications. The antibody is supplied in liquid form. It is Protein A/G purified from cell culture supernatant. Purity is >95% as determined by SDS-PAGE.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, NeA
• Reactivity: Human

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: BKX2299
• Purity: >95% as determined by SDS-PAGE.
• Purification: Protein A/G purified from cell culture supernatant.
• Endotoxin: Please contact with the lab for this information.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M PBS, pH 7.4.
• Concentration: Lot dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HC11, MCP-1, MCP1, Monocyte chemotactic protein 1, Monocyte chemotactic and activating factor, Small-inducible cytokine A2, SCYA2, MCAF, Monocyte secretory protein JE, C-C motif chemokine 2, Monocyte chemoattractant protein 1, CCL2

Flow-cytometry using anti-human CCL2 antibody. A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL2 antibody monoclonal antibody (Catalog # orb2960360, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CCL2 antibody. Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL2 antibody monoclonal antibody (Catalog # orb2960360, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for InVivoMAb Anti-Human CCL2/MCP-1.

Western blot analysis was performed using anti-CCL2/MCP-1 monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human CCL2/MCP-1 (Catalog No: orb2969181), 50 ng, Lane 2: recombinant human CCL2/MCP-1 (Catalog No: orb2969181), 30 ng, Lane 3: recombinant human CCL2/MCP-1 (Catalog No: orb2969181), 20 ng, Lane 4: recombinant human CCL2/MCP-1 (Catalog No: orb2969181), 10 ng.

Quick Database Links

UniProt

P13500