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Catalog Number | orb768137 |
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Category | Antibodies |
Description | Rabbit polyclonal antibody to Intestinal Cell Kinase (phospho-Tyr159) |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Immunogen | The antiserum was produced against synthesized peptide derived from human ICK around the phosphorylation site of Tyr159. AA range:125-174 |
MW | 71 |
Tested applications | ELISA, WB |
Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Anti-ICK antibody, anti-KIAA0936 antibody, anti-Se Read more... |
Note | For research use only |
Western Blot analysis of 3T3 cells using Phospho-Intestinal Cell Kinase (Y159) Polyclonal Antibody.
Western blot analysis of lysates from NIH/3T3 cells treated with starved 24h, using ICK (Phospho-Tyr159) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).