Interferon-inducible protein (IFITM3) Antibody (N-term)

• Catalog Number: orb1938125
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB13975
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: IF: 1:100~500, WB: 1:1000, WB: 1:2000, WB: 1:1000, IHC-P-Leica: 1:100, IHC-P-Leica: 1:100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 14632 Da
• Target: This Interferon-inducible protein (IFITM3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Interferon-inducible protein (IFITM3).
• UniProt ID: Q01628
• NCBI: NP_066362.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Interferon-induced transmembrane protein 3, Dispan
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-IFITM3 Antibody (N-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: 293 whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: NCI-H460 whole cell lysate. Lane 5: SK-BR-3 whole cell lysate. Lane 6: human placenta lysate. Lane 7: mouse colon lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 15 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of HeLa cells stained with IFITM3 (N-term) Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with Fragilis (IFITM3) (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min).

IFITM3 Antibody (N-term) western blot analysis in Hela, 293, A549, NCI-H460, HepG2, Sk-Br-3, A375 cell line lysates (35 ug/lane). This demonstrates the IFITM3 antibody detected the IFITM3 protein (arrow).

Immunohistochemical analysis on paraffin-embedded Human colon carcinoma tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary Antibody.

Immunohistochemical analysis on paraffin-embedded Human spleen tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary Antibody.

Western blot analysis of lysate from HT1080 cell line, using IFITM3 Antibody (N-term).diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary Antibody. Lysate at 35 ug per lane.