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Catalog Number | orb1228013 |
---|---|
Category | Proteins |
Description | Interferon gamma Recombinant Protein (HEK293 Expressed) |
Tested applications | WB |
Tag | Tag free |
Form/Appearance | Lyophilized |
Purity | > 90% as determined by SDS-PAGE. |
MW | 16.8 kDa |
Target | IFNG |
UniProt ID | P01579 |
Protein Sequence | Gln 24 - Gln 166 |
Source | HEK293 cells |
Biological Activity | The bio-activity of IFN-gamma was determined by dose-dependent inhibition of the proliferation of HT-29 cells. The EC50 for this effect is typically 0.41-0.74 ng/mL. |
NCBI | AAH70256 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | IFNG, IFG, IFI Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for WB. For research use only. |
Expiration Date | 6 months from date of receipt. |
Human IFN-gamma, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
98.00% | |
49.5 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
98.00% | |
49.5 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
98.00% | |
24.7 kDa (predicted). Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
98.00% | |
50.1 kDa (predicted). Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
98.00% | |
50.1 kDa (predicted). Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result. |