Interferon gamma (IFNG) Antibody

• Catalog Number: orb751169
• Category: Antibodies
• Description: Mouse monoclonal antibody against IFN gamma conjugated to Biotin
• Target: IFNG
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b
• Conjugation: Biotin
• Reactivity: Human
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab conjugated to Biotin
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Purification: Protein A
• Immunogen: Purified recombinant human full-length IFNG protein
• UniProt ID: P01579
• MW: 20-25kDa
• Tested applications: FC, IF, IHC
• Dilution range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Application notes: Positive Control: PMA stimulated human peripheral blood mononuclear cells. Renal or testicular carcinoma
• Clone Number: IFNG/466
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-AMCF1 antibody, anti-Beta thromboglobulin lik
• Research Area: Cancer Biology, Cardiovascular Research, Epigeneti
• Note: For research use only
• Entrez: 3458

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Interferon gamma Mouse Monoclonal Antibody (IFNG/466).

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Interferon gamma Mouse Monoclonal Antibody (IFNG/466).

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Interferon gamma Mouse Monoclonal Antibody (IFNG/466).

SDS-PAGE Analysis Purified Interferon gamma Mouse Monoclonal Antibody (IFNG/466). Confirmation of Purity and Integrity of Antibody.