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Catalog Number | orb2636422 |
---|---|
Category | Antibodies |
Description | Recognizes a protein of 20-25kDa, identified as human interferon. This MAb is specific to human IFN-g and recognizes both recombinant and native human IFN-gamma. T lymphocytes and NK cells mainly produce IFN-g. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | IFNG/9012 |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | A recombinant fragment (within amino acids 1-166) of human IFNG protein was used as the immunogen for the Interferon gamma antibody. |
Antibody Type | Primary Antibody |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml |
Conjugation | Unconjugated |
Formula | 1 mg/ml in 1X PBS; BSA free, sodium azide free |
Hazard Information | This Interferon gamma antibody is available for research use only. |
UniProt ID | P01579 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Application notes | Optimal dilution of the Interferon gamma antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human tonsil tissue with Interferon gamma antibody (clone IFNG/9012). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free IFNG antibody (clone IFNG/9012) as confirmation of integrity and purity.
IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FACS, ICC, IHC, IHC-Fr, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |