Interferon gamma Antibody

• Catalog Number: orb2636422
• Category: Antibodies
• Description: Recognizes a protein of 20-25kDa, identified as human interferon. This MAb is specific to human IFN-g and recognizes both recombinant and native human IFN-gamma. T lymphocytes and NK cells mainly produce IFN-g. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant fragment (within amino acids 1-166) of human IFNG protein was used as the immunogen for the Interferon gamma antibody.
• UniProt ID: P01579
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the Interferon gamma antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: IFNG/9012
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Interferon gamma antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human tonsil tissue with Interferon gamma antibody (clone IFNG/9012). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free IFNG antibody (clone IFNG/9012) as confirmation of integrity and purity.