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Catalog Number | orb750044 |
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Category | Antibodies |
Description | Recognizes a protein of 16-27 kDa, identified as human Interferon alpha 2 (IFNA2). Its epitope maps between amino acids 43-53 of the IFNA2. This mAb is specific for IFNA2 and does not cross-react with IFNA1. The epitopes of clones N27 and N39 mAbs are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFNA2 after viral infections. The IFNA family consists of 24 or more genes or pseudo-genes. The alpha interferons are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens. |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | Mouse IgG1, kappa |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Purity | Protein G affinity chromatography |
Immunogen | Purified recombinant human IFNA2 was used as the immunogen for the Interferon alpha 2 antibody. |
UniProt ID | P01563 |
Tested applications | ELISA |
Dilution range | ELISA (order BSA/sodium azide-free format for coating) |
Application notes | Optimal dilution of the Interferon alpha 2 antibody should be determined by the researcher. |
Antibody Type | Primary Antibody |
Clone Number | N27 |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Storage | Store the Interferon alpha 2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Hazard Information | This Interferon alpha 2 antibody is available for research use only. |
Note | For research use only |
SDS-PAGE Analysis of Purified, BSA-Free Interferon alpha 2 Antibody (clone N27). Confirmation of Integrity and Purity of the Antibody.