Integrin beta 4 Antibody / ITGB4 / CD104

• Catalog Number: orb248422
• Category: Antibodies
• Description: This antibody recognizes a protein of 205kDa, which is identified as integrin beta 4 (ITGB4) and CD104. Its epitope is localized in the extracellular domain of integrin beta 4 protein. Integrins are heterodimers comprised of <font face=symbol>a and <font face=symbol>b subunits, that are non-covalently associated transmembrane glycoprotein receptors. Different combinations of <font face=symbol>a and <font face=symbol>b polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha-6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: UM-A9
• Tested applications: Blocking, FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: The human squamous cell carcinoma line (UM-SCC1) was used as the immunogen for this Integrin beta 4 / CD104 antibody.
• Dilution range: Biological Blockade (Partially blocks laminin binding) (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Integrin Beta 4 antibody is available for research use only.
• Entrez: 3691
• Storage: Store the Integrin Beta 4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE analysis of Purified, BSA-Free Integrin Beta 4 antibody