Integrin beta 3 Antibody / ITGB3 / CD61

• Catalog Number: orb385996
• Category: Antibodies
• Description: Reacts with human CD61, also called Integrin beta 3 (GPIIIa, vitronectin receptor beta chain). It associates with the αV-chain (CD51) to form vitronectin receptor, or with the αIIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61). The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Phytohemagglutinin (PHA)-stimulated peripheral blood cells were used as the immunogen for the CD61 antibody.
• UniProt ID: P05106
• Tested applications: IHC
• Dilution range: Immunohistochemistry (Frozen & FFPE): 1-2ug/ml for 30 min at RT
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD1a antibody to be titered up or down for optimal performance.
• Antibody Type: Primary Antibody
• Clone Number: Y2/51
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This CD61 antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human spleen tissue with CD61 antibody (clone Y2/51). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free CD61 Antibody (clone Y2/51). Confirmation of Integrity and Purity of the Antibody.