Integrin beta 2 alpha subunit (CD11b/c, Mac-1) Antibody

SKU: orb3167401

Description

Our Anti-Integrin beta 2 alpha subunit mouse monoclonal primary antibody detects rat Integrin beta 2 alpha subunit, and is IgG. It is validated for use in FC, ICC, IHC-Paraffin-embedded, IP.

Images & Validation

• Tested Applications: FC, ICC, IHC, IP
• Dilution Range: IHC: 1:100-1:200; ICC: 1-2 µg/mL; IP: 1-5 µg/mL
• Reactivity: Rat
• Application Notes:
  FC: Flow Cytometry: Unfixed cells preferred, acetone fixed or quickly fixed 1% PLP fixed cells can be used. IHC: Immunohistochemical studies of rat fresh frozen tissue sections and paraffin-embedded tissue sections following either periodate-lysine-paraformaldehyde (PLP) fixation, or acetone. Works on very lightly PFA fixed, frozen tissues. (perfusion only 4% PFA 10-15' no post-fix). Epitope can be sensitive to fixation. Dilutions detection method dependent 1:100 to 1:200 recommended. ICC: Unfixed preferred, or acetone fixed cells; 5-10', 2% PLP fixed cells, 1-2 µg/mL. Dilution is detection method dependent. Immunoprecipitation: use rabbit anti-mouse or anti-mouse IgG beads for capture only. The use of Protein A or Protein G is not recommended. 1-5 µg/mL in restricted volumes. Clone does not work in traditional reduced Westerns. Use immunoprecipitation to resolve reactive protein bands.

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Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Clone No.: OX42, OX-42
• Immunogen: Rat peritoneal macrophages, whole cells. (Robinson, AP et al Immunology 1986 57 239-247)
• Target: Integrin beta 2 alpha subunit
• Molecular Weight: Immunoprecipitates three polypeptides of 160 kDa, 103 kDa, and 95 kDa and a fainter band may also be seen at 133 kDa under non-reducing conditions. If the immunoprecipitated proteins are reduced, two major peptides of 163 kDa and 100 kDa and a minor 135 kDa peptide are seen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized from PBS containing no preservatives.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD11b; CD11B; CD11 antigen-like family member B; ITGAM; Integrin beta 2 alpha subunit; CD11c;

Quick Database Links

Gene Symbol

Integrin beta 2 alpha subunit

UniProt

Q9JI30