Insulin antibody

• Catalog Number: orb344417
• Category: Antibodies
• Description: Insulin antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2D11.H5
• Tested applications: DOT, ELISA, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA).
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:25,000, IHC: 1:50 - 1:200, IP: User Optimized, WB: 1:50 - 1:200
• Form/Appearance: Liquid (sterile filtered)
• Purity: This protein A purified mouse monoclonal antibody reacts specifically with insulin from human and swine sources. Cross reactivity with insulin from mouse and rat does not occur. Cross reactivity with insulin from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P01308
• NCBI: P01308.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-insulin antibody, ILPR antibody, INS an
• Note: For research use only
• Application notes: Mouse Anti-Insulin Antibody has been tested by ELISA and dot blot and is suitable for immunohistochemistry, immunoblotting and immunoprecipitation. Insulin has a cytoplasmic localization. Pancreatic tissue or b-cells from islets of Langerhans can be used as a positive control.
• Expiration Date: 12 months from date of receipt.

Dot Blot analysis of ul of non-denatured human insulin at various dilutions starting at 1.0 ug/ml using Insulin antibody

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of using Insulin antibody