INSM1 antibody

SKU: orb2276425

Description

INSM1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:75-1:150
• Reactivity: Human
• Application Notes:
  Positive Control: Pancreas: Islet cells should show a moderate to strong INSM1 immunostaining Negative Control: Pancreas: All acinar cells should be negative Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-456R
• Immunogen: Recombinant fragment (around aa81-125) corresponding to the N-terminus of human INSM1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

A1; Insulinoma associated protein 1 (IA1); Zinc finger protein IA1

Moderate to strong INSM1 immunostaining in most cells of Langerhans islets of the pancreas.

Strong INSM1 immunostaining in a low grade neuroendocrine tumor of the appendix.

Strong INSM1 immunostaining of medullar but not cortical cells of the adrenal gland.

Quick Database Links

UniProt

Q01101