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Catalog Number | orb1938948 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB27636 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 112852 Da |
Target | This INPP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 950-979 amino acids from the C-terminal region of human INPP5B. |
UniProt ID | P32019 |
NCBI | NP_005531.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Type II inositol 1,5-trisphosphate 5-phosphatase, Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-INPP5B Antibody (C-term) at 1:1000 dilution + Daudi whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 113 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
INPP5B Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
INPP5B antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the INPP5B antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.